Toxicity of Cisplatin-Loaded Poly Butyl Cyanoacrylate Nanoparticles in a Brain Cancer Cell Line: Anionic Polymerization Results

Document Type: Research Articles

Authors

1 Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran

3 Department of Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

4 Department of Molecular Medicine, Shahid Beheshti University of Medical Sciences, School of Advanced Technologies in Medicine, Tehran, Iran

5 Department of Cell and Molecular Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

6 Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza

7 Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

8 Department of microbiology, parasitology and immunology, faculty of medicine, rafsanjan university of medical sciences, rafsanjan, Iran

9 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran

Abstract

 
Cancer is one of the most important issues in modern medicine and the most common cause of death after cardiovascular diseases in many countries. Brain cancer is one of the most common causes of cancer death among men and women, ranking third. Chemotherapeutic drugs that aim to prevent uncontrolled proliferation of cells in tissues of the body and induce apoptosis of tumor cells are prominent candidates for development. Since cisplatin has an apoptosis-inducing role, it is widely used as an anticancer agent. In this research, toxicity of cisplatin was studied with the C6 rat glioma cell lined using the MTT method. In addition, nanoparticles underwent SEM microscopic imaging. Particle average size, size distribution, polydispersity index (PDI) and zeta potential of poly butyl cyanoacrylate nanoparticles were found to be 222 nm, 0.470 ± 0.04 and 5.1 ± 0.2 mV, respectively. The results showed that nanoconjugates of cisplatin have more cytotoxic effects on C6 cells than the free drug (P<0.05), pointing to an enhanced potential of the synthesized nano-particles as a new nanocarrier for chemotherapy.

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