SNP and Haplotype Analysis of Vascular Endothelial Growth Factor (VEGF) Gene in Lung Cancer Patients of Kashmir

Naikoo, Niyaz A; Afroze, Dil; Rasool, Roohi; Shah, Sonaullah; Ahangar, A G; Bhat, Imtiyaz; Qasim, Iqbal; Siddiqi, Mushtaq A; Shah, Zafar

doi:10.22034/APJCP.2017.18.7.1799

SNP and Haplotype Analysis of Vascular Endothelial Growth Factor (VEGF) Gene in Lung Cancer Patients of Kashmir

Document Type : Research Articles

Authors

¹ Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura Srinagar, Kashmir, India.

² Department of General Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura Srinagar, Kashmir, India.

³ Department of Cardio Vascular Thoracic Surgery, Sher-I-Kashmir Institute of Medical Sciences, Soura Srinagar, Kashmir, India.

⁴ Advanced Centre for Human Genetics, Sher-I-Kashmir Institute of Medical Sciences, Soura Srinagar, Kashmir, India.

⁵ 5Islamic University of Science and Technology, Awantipora Pulwama J&K India.

10.22034/APJCP.2017.18.7.1799

Abstract

Vascular endothelial growth factor (VEGF) is a major mediator of angiogenesis involving tumor growth and metastasis. In this large case-control study, we investigated whether functional polymorphisms (+405C>G, +936C>T) in the VEGF gene are associated with the risk of lung cancer. The study investigates the association between variants of VEGF gene and lung cancer. We performed single nucleotide polymorphism (SNP), haplotype and linkage disequilibrium studies on 100 patients and 128 healthy controls with 2 SNPs in the VEGF gene. The results were analyzed using logistic regression models, adjusted for age and sex. No Significant association was detected between individual SNPs and lung cancer using all the models of inheritance (codominant, dominant, recessive, over dominant and additive) for finding an association between genotypes and the cancer risk. The P values obtained for two markers were non-significant (P>0.05). Haplotype analysis produced additional support for the non-association of individual haplotypes/ all haplotypes with the cancer risk (Global association P=0.56). Our findings suggest the non-involvement of genetic variants (+405C>G, +936C>T) of the VEGF gene in the etiology of lung cancer.

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