Haplotype Analysis of MDR1 and Risk for Cervical Cancer in Northeastern Thailand

Phuthong, Sophida; Settheetham-Ishida, Wannapa; Natphopsuk, Sitakan; Settheetham, Dariwan; Ishida, Takafumi

doi:10.22034/APJCP.2017.18.7.1815

Haplotype Analysis of MDR1 and Risk for Cervical Cancer in Northeastern Thailand

Document Type : Research Articles

Authors

¹ Department of Physiology, HPV and EBV and Carcinogenesis Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

² Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, Thailand.

³ Department of Environmental Health, Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand.

⁴ Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.

10.22034/APJCP.2017.18.7.1815

Abstract

Objective: The aim of this study was to investigate the association between genotype and haplotype of MDR1 (C1236T, G2677T/A and C3435T) and the risk for cervical cancer in Northeastern Thai women. Methods: An age-matched case-control study involving squamous cell cervical cancer (SCCA) patients (n=204) and healthy controls (n=204) was enrolled for MDR1 genotyping by real-time PCR method. Results: The genotype distribution of MDR1 in both patients and controls was not significantly different (p>0.05). The haplotype analysis showed that T-T-T was the most common haplotype in this population. Significantly increased risk of cervical cancer was observed in carriers of T-T-C and C-G-T haplotypes with ORs of 1.86 (95%CI=1.02-3.39, p=0.0416) and 2.00 (95%CI=1.18-3.40, p=0.0140), respectively. Analysis of 2677-3435 haplotype showed increased risk for cervical cancer in G-T (OR=1.55; 95% CI=1.12-2.13, p=0.0432) and T-C (OR=1.91; 95%CI=1.05-3.47, p=0.0325). Conclusion: The results provide evidence that haplotype of MDR1 may be an important risk factor for cervical cancer development in Northeastern Thai women.

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