Biomarkers in Tumorigenesis Using Cancer Cell Lines: A Systematic Review

Document Type: Systematic Review and Meta-analysis


1 Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, M.S.Ramaiah University of Applied Sciences, M S R Nagar, Bangalore, Karnataka, India.

2 Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, M.S.Ramaiah University of Applied Sciences, M S R Nagar, Bangalore, Karnataka, India.

3 College of Dental Medicine, Roseman University of Health Sciences, South Jordan, Utah.

4 Department of Maxillofacial surgery and Diagnostic Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.


Cancer is a leading cause of death worldwide. Despite many research advancements in the field, the genetic changes regulating the transformation of normal oral cells into malignant cells have not been fully elucidated. Several studies have evaluated carcinogenesis at the molecular level. Cancer cell lines are commonly used in biomedical research because they provide an unlimited source of cells and represent various stages of initiation and progression of carcinogenesis in vitro. Aims: The objective of the study was to review original research articles using cancer cell lines as a tool to understand carcinogenesis and to identify the genes involved in tumor development. Additionally, we also examined the application of the genes as predictive biomarkers. Methods and Materials: Several databases, including PubMed, Google Scholar, Ebsco, and Science Direct, were searched from 1985 to December 2016 using various combinations of the following key words: "mouth neoplasm", "cell lines", and "tumorigenesis". Original experimental studies published in English were included. We excluded letters to the editor, historic reviews, and unpublished data from the analysis. Results: There were 17 studies (in vitro) included in the analysis. There were 14 genes and 4 miRNAs involved in malignant transformation of oral keratinocytes into cancer cells. The most commonly studied genes were p53, cyclin D1, and hTERT. Conclusion: Additional reviews and studies are needed to identify a panel of genes specific to various potentially malignant disorders and to aid in the early detection of oral squamous cell carcinoma (OSCC) because tumorigenesis involves the mutation of multiple genes. Furthermore, improving advanced cost-effective diagnostic methods may benefit the public health sector.


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