Anthocyanins from the Fruit of Vitis Coignetiae Pulliat Inhibit TNF-Augmented Cancer Proliferation, Migration, and Invasion in A549 Cells

Document Type: Research Articles

Authors

1 Department of Internal Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

2 Pharmacology, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

3 Emergency Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.

4 Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan 614-052, Korea.

5 School of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, 660- 701, Korea.

6 Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Korea.

7 Surgery Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702, Korea.

Abstract

 
Objective: Anthocyanins belong to a class of flavonoids, exhibiting antioxidant and anti-inflammatory actions have been reported to have anti-cancer effects. Here, we investigated whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in human lung cancer A549 cells, which are critically involved in cancer metastasis. Methods: We used anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) which has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. We have performed cell proliferation assays, cell invasion assay, gelatin zymography, wound healing assay and western blotting to examine whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in A549 cells. Result: AIMs did not inhibit cancer cell proliferation on A549 cells. Also, AIMs suppressed cancer migration, and invasion by supressing MMP-2 and MMP-9 expression. The Immuno-blotting results also revealed that AIMs suppressed the proteins involved in cancer proliferation (COX- 2, C-myc, cyclin D1), migration and invasion (MMP-2, MMP-9), anti-apoptosis (XIAP, and c-IAP2), adhesion and angiogenesis (ICAM-1, VEGF). Conclusion: This study demonstrates that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit cancer proliferation, cancer migration, and invasion that is involve in cancer-metastasis. This study provides evidence that AIMs might have anti-cancer effects on human lung cancer.

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