Extraction and Characterization of Chick PEA (Cicer arietinum) Extract with Immunostimulant Activity in BALB/C MICE

Document Type: Research Articles

Authors

Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam Erode Dist-638401, India.

Abstract

Traditional plant medicines are used for a range of cancer conditions. The chickpea is highly proteinaceous and
consumed as a staple in many parts of the world. An evaluation of chemoprotective and immunomodulatory activities
of Cicer arietinum (CE) in cisplatin-induced immunosuppressed mice was here performed. Cisplatin was given
at 100mg/kg, intraperitoneally, and after induction of immunosuppression mice were treated with Cicer arietinum
(0.5 mg/dose/animal/IP) for a period of 10-day. The influence of the extract on lymphoid organ weight, bone marrow
cellularity, alpha esterase activity and on enzyme levels such as (SGOT,SGPT, Urea, Creatinine was assessed to
identify any chemoprotective influence. Administration of CE to cisplatin-treated mice alleviated the drastic reduction
in bone marrow cellularity and α- esterase positive cells seen with cisplatin. Thus myelosuppression was reversed or
inhibited. Cisplatin bids to DNA and causes damage resulting in chromosome breaks, micronucleus formation and cell
death. CE is comprised of numerous middle-chain aliphatic alcohols, aldehydes and ketones, in addition to compounds
like 7-hydroxy-1-methoxy-6-methylanthraquinone, cyclohexadecane (CAS) and 6-(amino methyl)-2-naphthol. These
latter are thought to contribute to the characteristic aroma of C. arietimnum. In conclusion, administration of CE
in cisplatin-treated mice, boosted bone marrow cellularity and increased α- esterase positive cells, thus reversing
myelosupproession.

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