Tsuchiya, Y., Loza, E., Villa-Gomez, G., Trujillo, C., Baez, S., Asai, T., Ikoma, T., Endoh, K., Nakamura, K. (2018). Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis. Asian Pacific Journal of Cancer Prevention, 19(4), 961-967. doi: 10.22034/APJCP.2018.19.4.961
Yasuo Tsuchiya; Ernest Loza; Guido Villa-Gomez; Carlos C Trujillo; Sergio Baez; Takao Asai; Toshikazu Ikoma; Kazuo Endoh; Kazutoshi Nakamura. "Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis". Asian Pacific Journal of Cancer Prevention, 19, 4, 2018, 961-967. doi: 10.22034/APJCP.2018.19.4.961
Tsuchiya, Y., Loza, E., Villa-Gomez, G., Trujillo, C., Baez, S., Asai, T., Ikoma, T., Endoh, K., Nakamura, K. (2018). 'Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis', Asian Pacific Journal of Cancer Prevention, 19(4), pp. 961-967. doi: 10.22034/APJCP.2018.19.4.961
Tsuchiya, Y., Loza, E., Villa-Gomez, G., Trujillo, C., Baez, S., Asai, T., Ikoma, T., Endoh, K., Nakamura, K. Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis. Asian Pacific Journal of Cancer Prevention, 2018; 19(4): 961-967. doi: 10.22034/APJCP.2018.19.4.961
Metagenomics of Microbial Communities in Gallbladder Bile from Patients with Gallbladder Cancer or Cholelithiasis
1Division of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
2Instituto de Gastroenterologia Boliviano-Japones, La Paz, Bolivia.
3Sotero del Rio Hospital, Santiago, Chile.
4Faculty of Medical Technology, Niigata University of Health and Welfare, Niigata, Japan.
5Faculty of Health and Medical Sciences, Hokuriku University, Kanazawa, Ishikawa, Japan.
6Faculty of Health Sciences, Niigata University of Health and Welfare, Niigata, Japan.
Receive Date: 20 September 2017,
Revise Date: 15 March 2018,
Accept Date: 05 March 2018
Abstract
Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. However, information on microbial pathogens in gallbladder bile of GBC patients is scarce. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). Seven GBC patients and 30 CL patients were enrolled in this study. Genomic DNA was extracted from bile and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database, and the bacteria were identified by homology searches and phylogenetic analysis. DNA was detected in the bile of three GBC (42.9%; Bolivia, 1; Chile, 2) and four CL patients (13.3%; Bolivia, 1; Chile, 3). Of the 37 patients, 30 (81.1%) were negative and unable to analyze. Salmonella typhi and Helicobacter sp. were not detected in bile from any GBC patients. As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enetrobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enerococcus gallinarum. Escherichia coli was detected in bile samples from both GBC and CL patients. Whether the bacteria detected in bile from GBC patients would associated with the development of GBC warrant further investigation.
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