Sirt1 Gene Expression and Gastric Epithelial Cells Tumor Stage in Patients with Helicobacter pylori Infection

Document Type: Research Articles

Authors

1 Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.

2 Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

3 Lung Diseases and Allergy Research Center,Kurdistan University of Medical Sciences, Sanandaj, Iran.

4 Cancer and Immunology Research Center,Kurdistan University of Medical Sciences, Sanandaj, Iran.

5 Department of Occupational Health Engineering, School of Health, Kurdistan University of Medical Sciences, Sanandaj, Iran.

6 Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

Abstract

Introduction: The World Health Organization has categorized Helicobacter pylori as a carcinogen for gastric
cancer, which causes human mortality worldwide. A number of studies have shown that H. pylori affects cell signaling
in gastric epithelial cells and changes the expression of some proteins such as proinflammatory cytokines. Bacterial
infections may alter sirt1 and sirt2 genes expression in inflammatory tissues and cancer cells. In this study, sirt1 and
sirt2 genes expression in gastric cancers was surveyed with reference to H. pylori status. Methods: Stomach biopsies
were collected from 50 gastric cancer patients, 25 H. pylori-positive and 25 H. pylori-negative as determined by the
urea rapid test. Tumor grade was determined by a pathologist. After total RNA extraction from gastric cancer biopsy
samples and cDNA synthesis, sirt1 and sirt2 genes expression levels were determined by Real Time PCR and ΔΔCT
methods. Results: There was no statistically significant link between H. pylori infection and sirt1 (P<0.899) and sirt2
(P<0.169) genes expression in gastric epithelial cells. However, pathologic findings showed that there is a statistically
significant relationship between sirt1 gene expression and the tumor grade (P<0.024). Discussion: A statistically
significant association was found between sirt1 gene expression and tumor grade of gastric cancers that could be due
to effects on progression of cancer cells infected with H. pylori.

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