Document Type: Research Articles
Department of Clinical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Colorectal Research Centre (CRRC), School of Medicine, Hazrat Rasool Hospital, Iran University of Medical Sciences and Health Care Services, Tehran, Iran.
Department of Urology, School of Medicine, Hazrat Rasool Hospital, Iran University of Medical Sciences and Health Care Services, Tehran, Iran.
Background: Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. Despite recent progress in
diagnosis and treatment, it remains a major health problem and further studies are needed. We here investigated expression
profiles of the FBXO39, ETS-1 and BMI-1 genes in CRCs to validate any possible diagnostic/prognostic significance.
Material and Methods: Thirty six patients with locally advanced CRC admitted to Hazrate-Rasoul Hospital-Tehran
were enrolled. Initially the expression pattern of FBXO39, ETS-1 and BMI-1 genes were determined using RT-PCR
in CRC tumor and adjacent normal tissues then real-time RT-PCR was employed to quantify BMI-1 gene expression.
Results: FBXO39 expression was restricted to tumor tissues. Interestingly, expression of this gene was detected in all
stage-0 tumor samples. There was a significant relation between FBXO39 gene expression and lymph node involvement.
The ETS-1 gene was expressed in 66% of all tumor tissues with p-value=0.03 for increase as compared to the adjacent
normal samples. In addition, there was a significant relation between ETS-1 gene expression and tumor size and lymph
node involvement. RT-PCR demonstrated BMI-1 gene expression in both tumor and normal tissues and quantification
by real-time RT-PCR showed no association between BMI-1 levels and CRC clinicopathological features. Conclusion:
Expression of FBXO39 and ETS-1 with lymph node involvement may be considered as an alarm for the occurrence
of CRC metastasis, and therfore have prognostic value while BMI-1 appears without importance.