Prognostic Value of FBXO39 and ETS-1 but not BMI-1 in Iranian Colorectal Cancer Patients

Document Type: Research Articles

Authors

1 Department of Clinical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

2 Colorectal Research Centre (CRRC), School of Medicine, Hazrat Rasool Hospital, Iran University of Medical Sciences and Health Care Services, Tehran, Iran.

3 Department of Urology, School of Medicine, Hazrat Rasool Hospital, Iran University of Medical Sciences and Health Care Services, Tehran, Iran.

Abstract

Background: Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. Despite recent progress in
diagnosis and treatment, it remains a major health problem and further studies are needed. We here investigated expression
profiles of the FBXO39, ETS-1 and BMI-1 genes in CRCs to validate any possible diagnostic/prognostic significance.
Material and Methods: Thirty six patients with locally advanced CRC admitted to Hazrate-Rasoul Hospital-Tehran
were enrolled. Initially the expression pattern of FBXO39, ETS-1 and BMI-1 genes were determined using RT-PCR
in CRC tumor and adjacent normal tissues then real-time RT-PCR was employed to quantify BMI-1 gene expression.
Results: FBXO39 expression was restricted to tumor tissues. Interestingly, expression of this gene was detected in all
stage-0 tumor samples. There was a significant relation between FBXO39 gene expression and lymph node involvement.
The ETS-1 gene was expressed in 66% of all tumor tissues with p-value=0.03 for increase as compared to the adjacent
normal samples. In addition, there was a significant relation between ETS-1 gene expression and tumor size and lymph
node involvement. RT-PCR demonstrated BMI-1 gene expression in both tumor and normal tissues and quantification
by real-time RT-PCR showed no association between BMI-1 levels and CRC clinicopathological features. Conclusion:
Expression of FBXO39 and ETS-1 with lymph node involvement may be considered as an alarm for the occurrence
of CRC metastasis, and therfore have prognostic value while BMI-1 appears without importance.

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