Document Type: Research Articles
Biochemistry Department, Faculty of Science, Ain Shams University, Egypt.
Biochemistry Department, National Center for Radiation Research and Technology Atomic Energy Authority, Egypt.
Biological sciences Department, Faculty of Science, Beirut Arab University, Lebanon.
Biochemistry Department, Faculty of Science, Alexandria University Alexandria, Egypt.
Pharmaceutical and Fermentation Industries Development Centre, City for Scientific Research and Technology Applications, Alexandria, Egypt.
Introduction: Colon Cancer remains one of the major worldwide causes of cancer related morbidity and mortality
in both genders. Berberine (BBR), a major component of alkaloids that possess a variety of pharmacological properties.
Objective: This study shows the ameliorating roles of berberine on 1,2 Di methyl hydrazine (DMH) induced colon
cancer in male Swiss albino rats. Methods: The rats were segregated into four groups: group 1, control rats; group
2, rats were orally received berberine (75 mg/kg b.wt./day) daily for ten weeks; group 3,rats were subcutaneously
injected with DMH (20 mg/kg b.wt) once a week for 8 weeks ,group 4, rats were treated firstly with berberine for
two weeks before DMH intoxication and concurrently with DMH over 8 weeks. Result: DMH injection decreased
the antioxidants levels (GSH and SOD) and increased inflammatory markers (MPO, MAPK and COX-2). Moreover,
it downregulated apoptotic markers (Caspase-3 and P53) expression that confirmed by colon cell proliferation. The
prophylactic effect of berberine was noticed as its pre-and co-administration increased antioxidants status and apoptotic
markers expression that associated with inflammatory markers down-regulation with absence of proliferated colon
cells. Conclusion: Therefore, the overall findings proved that the anti-proliferative effect of berberine return to its
antioxidants and anti-inflammatory properties that activated the programmed cell death process.