Document Type: Research Articles
Sorbonne University, UPMC Univ. Paris 06, École Normale Supérieure, PSL Research University, CNRS, INSERM, APHP, Laboratoire des Biomolécules (LBM), Paris, France.
Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
Objective: This study investigated the DNA promoter methylation profiles of BRCA1, RASSF1A and GSTP1 genes,
both individually and in an integrative manner in order to clarify their correlation with clinicopathological parameters of
breast cancer from Vietnamese patients, and establish new potential integrative methylation biomarkers for breast cancer
detection. Material and methods: The methylation frequencies of BRCA1, RASSF1A and GSTP1 were analyzed by
methylation specific polymerase chain reaction (MSP) in 70 specimens of breast carcinomas and 79 pairs of tumor and
matched adjacent normal tissues from breast cancer patients. Results: All the three analyzed genes showed a concordance
concerning their promoter methylation in tumor and adjacent normal tissue. The methylation of BRCA1, RASSF1A
and GSTP1 was found in 58.23 %, 74.68 % and 59.49 % of tumor tissues and 51.90 %, 63.29 % and 35.44 % of
corresponding adjacent tissues, respectively. When each gene was assessed individually, only the methylation of
GSTP1 was significantly associated with tumor tissues (p=0.003). However, the methylation frequency of at least one of
the three genes and the methylation frequency of all the three genes both showed significant association with tumor
(p=0.008 and p=0.04, respectively). The methylation of BRCA1 was found to be significantly associated with tumor
grade (p=0.01). Conclusion: This study emphasized that the panel of the three genes BRCA1, RASSF1A and GSTP1
can be further developed as potential biomarkers in diagnosis and classification of breast cancer in Vietnamese women.