Document Type: Research Articles
University of Uberaba, Uberaba, MG, Brazil.
Cefores, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
Centro Universitário do Triângulo, Uberlândia, MG, Brazil.
São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.
Aim: to compare the levels of IFN-γ, TGF-β and C-reactive protein (CRP) in healthy patients (HP) and chronic
periodontitis patients (CP) before and seven days after the last session of Non-Surgical Periodontal Treatment (NSPT).
Materials and Methods: 40 subjects were divided into two groups: healthy (n= 20), and with chronic periodontitis
(n = 20). Serum and gingival crevicular fluid (GCF) were collected from each patient and quantified for IFN-γ,
TGF-β and CRP using the enzyme-linked immunosorbent assay (ELISA). Results: IFN-γ was found to be higher in
the GCF of the CP group before NSPT in relation to the HP group (p<0.05), and it had significant higher levels after
seven days of NSPT (p<0.05). The levels of TGF-β in the GCF of CP patients before NSPT were significantly higher
when compared to HP (p<0.05), but they decreased after seven days of NSPT (p>0.05). Serum CRP levels did not show
statistical difference between CP and HP before or after NSPT. Conclusion: Therefore, our results demonstrated for
the first time that NSPT causes early exacerbation of the immune response at the local level represented by increased
levels of IFN-γ and decreased levels of TGF-β in the gingival crevicular fluid after seven days of treatment.