Document Type : Research Articles
Department of Biotechnology, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Bannari Amman Institute of Technology, Sathyamangalam, India.
Background: The intendment of this study is to determine the pursuance in – vitro anticancer activity and
cytotoxicity of Syzygium aromaticum against the human cervical cancer cell line (HeLa) compared to the normal cell
lines. Apoptogenic properties of DCM extract of Eugenol was determined in this entire study. Materials and Methods:
HeLa cell lines were cultured in DMEM medium and incubated with different concentration of DCM – Eugenol
extract. MTT assay brought out the way to determine the cell viability and quantification was done with the optical
absorbance at 570 nm and 620 nm as reference. Apoptotic cells were affirmed by dual staining using acridine orange
bromide. Besides, the morphology of the nucleus was also confirmed by dual staining. Eugenol inhibited 50% growth
(IC50) of HeLa cell lines at 200 mg/ml of extract concentration. Results: Inhibitory efficacy of eugenol isolated from
Syzyzgyium aromaticum showed the cell – viability in time and dose dependent manner with consistent morphological
changes. Flow cytometer determined the apoptosis confirming the cytotoxicity value for MTT at IC50 with 81.85% cell
viability. Dual staining firmly enacts the damaged cells due to AO indicating apoptosis confirmation by dual staining.
Morphological analysis also clearly states that nil apoptosis has been seen in control and similarly in eugenol treated
when compared to cancerous HeLa cell – line. Conclusion: Evaluation of cytotoxicity effect of eugenol isolated from
Syzygium aromaticum showed it can be unrivalled dormant source of prodigious changes in HeLa cell line indicating
(revealing) that chemotherapeutic agent.