Comparative Study of Correlation between Angiogenesis Markers (CD31) and Ki67 Marker with Behavior of Aggressive and Nonaggressive Central Giant Cell Granuloma with Immunohistochemistry Technique

Document Type : Research Articles


Dental Material Research Center, Department of Oral and Maxillofacial, Dental School, Isfahan University of Medical Sciences, Isfahan, Iran.


Background: The central giant cell granuloma (CGCG) is generally considered a non-neoplastic lesion. However,
some cases show aggressive behavior like neoplasms. Based on clinical observations, a number of researchers have
classified this lesion into aggressive and non- aggressive types. This study was aimed to investigate the association
between clinical behavior and histopathological features using immunohistochemical vascular CD31 and cellular
proliferation Ki67 markers. Materials and methods: In this descriptive-analytical, clinicopathological and
immunohistochemical study, 50 CGCGs, including 25 aggressive and 25 non-aggressive types were selected according
to Chuong’s classification. The samples were then subjected to immunohistochemical staining to analyze positivity for
CD31 and Ki67 markers. Numbers of blood vessels and percentage proliferation of underlying fibroendothelial cells
were assessed, and the obtained results were analyzed with the t-test and the Mann-Whitney test. Results: The results
showed a significant difference between aggressive and non-aggressive CGCG lesions in the mean incidences of Ki67
(p=0.044). and CD31 (p=0.003) positivity. Conclusion: The present evaluation of expression rates for the vascular
CD31 and cellular proliferation Ki67 markers showed there might be a positive relation between the clinical features
and histopathology of CGCG. Furthermore, clinical behavior may be predicted based on features such as the number
of blood vessels and proliferation of fibroendothelial cells.


Main Subjects

Volume 19, Issue 8
August 2018
Pages 2279-2283
  • Receive Date: 17 March 2018
  • Revise Date: 24 May 2018
  • Accept Date: 29 June 2018
  • First Publish Date: 01 August 2018