Nisin Induces Cytotoxicity and Apoptosis in Human Asterocytoma Cell Line (SW1088)

Document Type: Research Articles

Authors

1 Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

2 Molecular Medicine Research Center, Research Institute of Basic Medical Science, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

3 Departments of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

4 Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

5 Departments of Clinical Biochemistry, َAfzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

6 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

Abstract

Background: Nisin is a member of the group of anti-microbial peptides which are considered as bacteriocins,
but it possesses a vast range of activities. Astrocytoma is among the most prevalent types of brain tumor globally.
Considering all facts about this peptide, the aim of the present study was the evaluation of any impact of nisin on
proliferation and apoptosis of an astrocytoma cell line (SW1088). Methods: The SW1088 cell line was purchased from
the Pasteur Institute of Iran and treated with various concentrations of Nisin. Nisin-induced cell toxicity and apoptosis
were detected by both MTT assay and annexin V-FITC /propidium iodide (PI) staining. Result: In current study we
observed that the cell death and apoptosis were significantly increased following nisin treatment, as compared to the
control group. Conclusion: These results open a new window for establishment promising approaches with the concept
of anti-cancer therapy by nisin in the future.

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