Document Type : Research Articles
Department of Medical Lab Technology, Prince Fahd Bin Sultan Research Chair, Faculty of Applied Medical Sciences,University of Tabuk, Tabuk, Kingdom of Saudi Arabia.
Department of Surgical Oncology, Faculty of Medicine University of Tabuk, Tabuk, Kingdom of Saudi Arabia.
Aim: microRNA-423 is an oncogenic factor which is frequently upregulated in cancer. However, associations with
breast cancer risk remain inconsistent. Therefore, we investigated the prevalence of microRNA-423 rs6505162C>T
gene variation with breast cancer susceptibility in Saudi women. Methodology: This study was conducted on 100
breast cancer patients and 124 matched healthy individuals. Genotyping of the microRNA-423 rs6505162C/T gene
variation was performed by using the amplification refractory mutation system PCR method (ARMS-PCR). Results:
A significant difference was observed in the genotype distribution between the breast cancer cases and controls
(p=0.0001), the frequencies of the genotypes CC,CT and TT being 25%, 52% and 23% in patients and 65%,20% and
15% respectively, in controls. The microRNA-423 C>T variant was associated with an increased risk of breast cancer
in codominant models for (OR = 6.73, 95 % CI, 3.50-12.97; RR 2.35(1.67-3.30, p=0.0001) the microRNA-423TT
genotype and (OR = 4.14, 95 % CI, 1.93-8.87; p=0.0003) microRNA-423CT (OR= 6.73, 95% CI, 3.50-12.97; p=0.0001)
and also with the dominant model (OR 5.6(3.14-1.01), p=0.0001) CT+TT vs CC) with a non-significant association for
the recessive model (OR=1.75, 95%CI=0.08-3.44, P=0.139, TT vs CC+CT). The T allele significantly increased the
risk of breast cancer (OR =2.63, 95 % CI, 1.77-3.91; p=0.001) compared to the C allele. Some 6.73 ,4.14 and 2.63 fold
increased risk of developing breast cancer was associated with TT and CT genotypes and the T allele of microRNA-423
in the northwestern region of Saudi Arabia. Conclusion: Our findings indicate that the microRNA-423 TT genotype
and the T allele are associated with an increased susceptibility, metastasis and advanced stage of breast cancer in Saudi
Arabian patients. Further studies with larger sample sizes are necessary to confirm our findings.