Cytotoxicity Enhancement of Paclitaxel by Loading on Stearate-g-dextran Micelles on Breast Cancer Cell Line MCF-7

Document Type: Research Articles

Authors

1 Department of Biology, College of Basic Science, Hamedan Branch, Islamic Azad University, Hamedan Branch, Hamedan, Iran.

2 Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

3 Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

4 Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

5 Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

6 Department of Pharmaceutics, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

Abstract

Objective: Paclitaxel (PTX) is a chemotherapeutic agent used for treating breast cancer. The study aimed to prepare
PTX loaded dextran stearate (Dex-SA) and evaluate its efficacy against human breast cancer cell line MCF-7. Methods:
Dex-SA/PTX micelles were prepared by dialysis method. The micelles size, zeta potential and particle size distribution
were measured by dynamic laser light scattering method. Amount of loaded PTX on the polymer measured by HPLC.
Release profiles of the drug from the micelles were obtained in buffer (phosphate pH=7.4). Then the cytotoxicity of blank
micelles, Dex-SA/PTX micelles and free PTX were evaluated in the MCF-7 cells by MTT method. Result: Loading
efficiency of PTX on the Dex-SA was measured about 84.24±9.07%. The smallest particles size was about 193.9±7.1
nm but the other formulation with larger particle size had better zeta potential (-33.5±6.74 mV). The drug release
from the micelles was slowly and reached steady state after about 12 hours. The cytotoxicity experiment showed that
Dex-SA/PTX micelles have more cytotoxicity compared to free PTX against MCF7 cell lines. Conclusions: Dex-SA
polymeric micelle is a suitable carrier for hydrophobic cytotoxic drugs such as PTX.

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