Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

Document Type: Research Articles

Authors

1 Infectious and Tropical Disease Research Center Health Research Institute; Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Department of Surgery, Imam Khoeini hospital, , Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Department of Pathology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

5 Infectious Diseases Department, Razi Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

6 Biostatistic Department, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background: Ductal carcinoma is one of the most common breast cancer (BrC) among the women in the world.
Several factors may involve in establishment of breast cancer. The role of viral infections have been investigated in
BrC, Among them the association of Epstein Barr virus have been reported in the patients with breast cancer type
ductal carcinoma. Thus this study was conducted to evaluate the rate of Epstein Barr virus in women with breast cancer
type ductal carcinoma. Material and methods: A total of 72 formalin-fixed paraffin-embedded tissue blocks samples
were collected from 37 (51.38%) women with breast cancer type ductal carcinoma and 35 (48.61%) samples of breast
with fibro adenoma as control group. The DNA was extracted for all the samples. The detection of EBNA 3C EBV
DNA was done by nested PCR. The results of positive were sequenced to confirm PCR product and determine EBV
genotypes. Results: About 10/37 (27.02%) samples of ductal breast carcinoma were showed positive for EBNA 3C
EBV DNA while 4/35 (11.42%) of fibro adenoma were positive for EBNA 3C EBV DNA (p= 0.095). Randomly 7
PCR products were sequenced and the results of sequencing EBNA 3C shows, the detected EBVDNA were type 1
EBV type. Conclusion: This study shows high prevalence of 27.02% EBV DNA type 1 was found in formalin-fixed
paraffin-embedded tissue of Patients with ductal breast carcinoma. The outcomes of this study suggesting that EBV
might have a significant role in breast cancer in Ahvaz city, south west region of Iran. However the expression of EBV
oncoproteins ,EBNA1, LMP1, and LMP2 require to be determined with ductal carcinoma cells. About 72.97% breast
samples showed negative for EBVDNA. The role other viruses including Human cytomegalovirus, papilloma viruses
and Merkel viruses are required to be investigated in further studies.

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