Document Type: Research Articles
Departamento of Investigacion, Laboratorio Central ADN SA de CV, Morelia, Michoacán, Mexico.
Cambrico Biotech, Sevilla, Spain.
Cambrix Genomic Institute, Sevilla, Spain.
Background: Human papillomavirus (HPV) subtypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 have been
implicated in the development of cervical cancer (CC). These 13 high risk HPV types have been shown to be present
in up to 99.7% of CC samples. In Mexico, this cancer is the leading cause of death from malignancy among women.
The aim of this study was to determine the prevalence of different HPV genotypes and investigate epidemiological aspects
associated with HPV infection in women from Cozumel. Material and methods: We performed an epidemiological,
prospective and cross sectional study with 1,187 who accepted participation in a campaign of screening for CC, during
the period 2014 to 2015. Data on epidemiological and socio-economic variables were obtained. Cervical cells were
collected for detection of HPV DNA and typing of HPV-positive samples by Multiplex PCR, using a commercial
kit for 16 viral genotypes. Results: The overall prevalence of HPV in women from Cozumel was 15.8 % (188/1,187),
either single (13.6%) or multiple (2.19 %). The most common HPV types , in descending order of frequency, were 58
(24.5 %), 59 (13.3 %), 39 (12.2 %) and 66 (9.6 %). The most frequent high risk types were HPV-58 and -59 and of low
risk HPV types the most common was HPV-6. Number of sexual partners (OR=4.78; 95% CI= 2.73-8.37; P=<0.0001)
and age of first coitus (OR=0.51; 95% CI=0.32-0.81; P=<0.0011) were significantly associated with HPV infection.
Conclusions: Our data indicate that the overall incidence of high risk HPV infection in Cozumel is low as compared to
other studies worldwide, with a different profile of subtypes. However, as expected, risky sexual behavior was found
associated with positive cases of HPV. These results highlight the need for establish strategies to prevent HPV acquisition
and evaluate the impact of a vaccine application in the Cozumel population.