Expression Analysis of Serum microRNA-34a and microRNA-183 in Hepatocellular Carcinoma

Document Type: Research Articles

Authors

1 Department of Medicine, Maulana Azad Medical College, New Delhi, India.

2 Department of Pharmacology, Faculty of Medicine, Wasit University, Iraq.

3 Department of Bioengineering and Technology, Gauhati University, Assam.

4 Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.

5 Department of Medical Toxicology, Jamia Hamdard, Delhi, India.

6 Department of Biological Sciences, Jamia Millia Islamia, New Delhi, India.

Abstract

Background/objective: HCC is a multistep process starting from chronic hepatitis that progress through cirrhosis
to HCC. MicroRNA expression level was found to be deregulated in HCC. To find out whether the expression level of
miR-34a and miR-183 was deregulated in HCC compared to controls without HCC. Methods: Real time quantitative
PCR was done to find out the miRNA expression level in terms of Ct value followed by statistical analysis. Results:
Over-expression of miR-183 and under-expression of miR-34a in HCC was detected. All changes in expression level
of miR-34a and miR-183 were found to be due to HCC compared to controls without HCC. So both miR-34a and
miR-183 were suitable to differentiate HCC from Cirrhosis and chronic hepatitis with an efficient diagnostic power of
sensitivity, specificity and expression level. But they might not have any role in patients’ survival. Conclusion: miR-
34a and miR-183 might be considered as potential markers of HCC screening molecule in addition to other approved
panel of marker. Our study warrants further expression level study.

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