GSTM1 and GSTT1 Polymorphisms and Susceptibility to Prostate Cancer: A Case-Control Study of the Algerian Population

Document Type: Research Articles

Authors

1 Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Algeria.

2 Private Medical Cabinet of Uro-Surgery, Annaba, Algeria.

Abstract

Objective: Prostate cancer (PCa) is a major public health problem worldwide, with high morbidity and mortality
levels. Advanced age, androgen stimulation, and ethnicity have been reported to be possible risk factors. It has been
suggested that particular genetic polymorphisms in glutathione S-transferases (GST), xenobiotic-metabolising enzymes,
could predispose to prostate cancer through heritable deficiency in detoxification of environmental carcinogens.
Conflicts in the published results and the absence of similar in depth studies in Algeria prompted us to perform the
present case-control study of GSTM1 and GSTT1 polymorphisms and their possible association with PCa in an Algerian
population. Methods: We determined GSTM1 and GSTT1 genotypes for 49 histologically verified prostate cancer
patients and in 41 age-matched healthy controls by multiplex polymerase chain reaction (PCR) using peripheral blood
DNA samples. Result: While an association between the GSTM1 null genotype and PCa risk (OR= 3.69, 95% CI=
1.30-10.44; P = 0.01) was evident, the GSTT1 null genotype (OR= 0.92, 95% IC= 0.32-2.62; P = 0.49) appeared without influence. Furthermore, no statistically significant differences between the double null genotype and PCa is detected, also no statistically significant differences between smoking status and PCa is detected. Conclusion: The GSTM1 null genotype may increase individual susceptibility to prostate cancer. On the other hand, the null-activity genotype of
GSTT1 did not appear to contribute to the risk of prostate cancer in our population.

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