Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) Polymorphisms and Lung Cancer Risk among a Select Group of Iranian People

Document Type: Research Articles

Authors

1 Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Khorramabad, Iran.

2 Pulmonary Department, Lorestan University of Medical Science, Khorramabad, Iran.

3 Iran National Tumor Bank, Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.

4 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Abstract

Objective(s): Lung cancer, caused primarily by smoking, is one of the leading determinants of mortality throughout
the world. Here we investigated the effects of polymorphisms in two enzymes, i.e., GSTT1 and GSTM1, related to
the antioxidant defense line against carcinogens associated with lung cancer among a select group of Iranian people.
Materials and Methods: One hundred and twenty lung cancer patients from two referral centers in Tehran, Iran, were
recruited for comparison with 120 healthy controls. Genomic DNA was extracted from the FFPE tumor tissues of
the select cases and peripheral blood buffy coats of healthy controls. The polymorphisms of GSTT1 and GSTM1 were
investigated by multiplex polymerase chain reaction. Results: With the 240 samples studied, no specific relationship
with lung cancer was discerned for the GSTM1 (P=0.35; OR=1/33; 95% CI=0.79-2.25) polymorphism, but the GSTT1
(P=0.005; OR=2.4; CI=1.32-4.35) gene polymorphism revealed a notable association on logistic regression, taking
into account age and sex factors. Furthermore, the GSTT1 genotype distribution in patients with LSCC was different
from that of healthy cases (P=0.006; OR=3.11; CI=1.38-7.04). The risk of developing lung cancer with the T0M1
genotype was 3.46 times higher than with T1M1 genotype (P=0.002; OR=3.46; CI=1.61-7.46). Moreover, the risk of
developing LSCC cancer in people with T0M1 genotypes was significantly elevated (P=0.004; OR=4.5; CI=1.62-12.52).
Conclusion: Unlike GSTM1, the GSTT1 genotype distribution is associated with the incidence of lung cancer in Iranian
people. Different types of lung cancer appear to show various correlations with GST polymorphisms in this regard.

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