Association of Glutathione-S-Transferases M1 and T1 Deletional Variants with Development of Oral Squamous Cell Carcinoma: A Study in the South-East of Iran

Document Type: Research Articles

Authors

1 Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.

2 Students Scientific Research Center, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.

3 Clinical Research Development Unit, Amir-Al-Momenin Hospital, Zabol University of Medical Sciences, Zabol, Iran.

4 Cardiovascular Diseases Research Center and Department of Molecular Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.

Abstract

Background: The role of genetic polymorphisms in genes of Glutathione-S-transferases (GST) enzymes in susceptibility
to oral cavity cancers is controversial. Oral Squamous Cell Carcinoma (OSCC) is the most common oral cavity neoplasm.
Aimed to evaluate the potential impacts of two well-known null variants residing in the gene encoding GSTM1 and
GSTT1 enzymes of OSCC patients in the southeast of Iran. Methods: In a case-control design, 113 individuals (50
OSCC patients, and 63 healthy subjects) were included. DNA was extracted using paraffin-embedded tissues. GST
genotyping was carried out using multiplex PCR. Results: In 113 participants, 41 (36.3%) and 72 (63.7%) were males
and females respectively. No significant difference was recognized for distribution of GSTM1 (P=0.11) and GSTT1
(P=0.28) null genotypes between OSCC patients (58%, and 24% respectively) and healthy controls (42.9% and 15.9%
respectively). Also, no significant difference was noted regarding the frequency of GSTM1 null genotype in different
histological grades, however, those patients with more aggressive disease (poorly differentiated or grade III) revealed
with a significantly higher ratio (66.7%) of GSTT1 null genotype (P=0.002). The highest odds ratio for OSCC was related
to combined null genotypes for GSTM1 and GSTT1 (OR=2.5, 95% CI: 0.7-9.2), however, this was not statistically
significant finding (P=0.15). Conclusion: Null genotypes polymorphisms were more common in OSCC than healthy
individuals. GSTT1 null genotype may be an important genetic factor in the progression of OSCC.

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