The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant Lesions

Document Type : Research Articles

Authors

1 Institute of Immunology and Infectious Diseases, Iran University of medical sciences, Tehran, Iran.

2 Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran.

3 Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz Iran.

4 Department of Pathology and Laboratory Medicine, Iran University of Medical Sciences, Tehran, Iran.

5 Firoozgar Clinical Research Development Center (FCRDC), Iran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorial
subject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV)
as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it in
tissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genome
and its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue. Method:
in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects had
healthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1
gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics software
CLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations. Result: a total of 157 subjects were
participated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched by
the cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66,
respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencing
analysis showed all were HBoV-1 genotype. Conclusion: our study showed there are low rate of HBoV genome in
Iranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1
according to phylogenetic analysis.

Highlights

 

Keywords

Main Subjects

Full Text

 

References

 
Asian Pacific Journal of Cancer Prevention
Volume 19, Issue 11
November 2018
Pages 3295-3299

Files

History

  • Receive Date: 23 July 2018
  • Revise Date: 09 September 2018
  • Accept Date: 14 October 2018

Share

How to cite

Statistics