Comparison of Salivary and Serum Soluble CD44 Levels between Patients with Oral SCC and Healthy Controls

Document Type: Research Articles

Authors

1 Dental Materials Rresearch Center, Health Research Institute, Babol University of medical sciences, Babol, Iran.

2 Department of Oral and Maxillofacial Surgery, School of Dentistry, Babol University of medical sciences, Babol, Iran.

3 Students Research Committee, Babol University of medical sciences, Babol, Iran.

4 Cellular and Molecular Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

5 Non-Communicable Pediatrics Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

6 Department of Oral and Maxillofacial Diseases, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.

7 Department of Medical Physics, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

8 Department of Immunology, Babol University of Medical Sciences, Babol, Iran.

Abstract

Background: The most common type of oral cancer is oral squamous cell carcinoma. If it is diagnosed in the early
stages; the success of the treatment can be increased. It seems that ELISA-based techniques as a screening tool for
society are the most cost-effective methods for early diagnosis. CD44 is a key marker for the detection of SCC stem
cells. The aim of this study was to compare the level of soluble CD44 in saliva and serum between patients with oral
SCC and healthy controls. Materials and Methods: Saliva and serum were collected from 20 patients with primary
OSCC and 20 healthy persons as control group. The samples were evaluated by an ELISA test kit. Data were analyzed
by SPSS software version 22, chi-square, ANOVA, T-test and Spearman correlation test. Results: The mean of soluble
CD44 level in serum and saliva of the patient and control groups are 531.51±228.95 and 453.3±113.74 (for serum)
and 48.53±59.02 and 17.76±39.14 (for saliva) respectively. There was no statistically significant difference in serum
and saliva solCD44 level between the patient and control groups (P value = 0.182 and P value = 0.061 respectively).
Also, there was no significant correlation between the solCD44 level in each patient and control group in serum (P
value = 0.61) and in saliva (P value = 0.445). Conclusions: Determination of solCD44 level in saliva and serum can
be a useful method for diagnosis the person’s involvement with cancer cells and the cancer in the early stages. But
according to the controversial outcomes of past studies, larger and more accurate studies are needed in groups with
more cases of oral cancer.

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