MiR-126 Modulates Angiogenesis in Breast Cancer by Targeting VEGF-A -mRNA

Document Type: Research Articles

Author

Department of Biology, College Education for Pure Sciences, Thi-Qar University, Nasiriya, Iraq.

Abstract

Background: Breast cancer is most serious reasons of women death around worldwide result in increasing its
morbidity and mortality. MicroRNAs are considered as significant regulators of cancer biological processes. The main
aim of this study is restoration of miR-126 could lead to modulate breast cell line and impairs their proliferation by
targeting vascular endothelial growth factor gene (VEGF-A). Methods: Breast cancer cell line (MCF7) was transfected
by miR-126 lipofectamine and negative miR control for 24 hr. Cytotoxic effects of miR-126 lipofectamine were
determined by cell viability assay. Cell proliferation and cell cycle were quantitatively measured using PicoGreen
assay and DAPI stain-flow cytometer analysis. For further investigation, Taq-Man real time PCR assay was performed
to detect relative VEGF-A and miRNA-126 level. Results: MiR-126 was overexpressed in treated breast cancer cell
(MCF7) compared with control cells. miR-126 expression has been associated –with a decrease in cell proliferation
and arrested MCF7 cells at G1 phase. The study found that vascular endothelial growth factor is regulated by miR-
126. Hence, VEGF-A is considered as functional vital and direct target to miR-126 in breast cancer cell line (MCF7).
Conclusions: This study provided that manipulated miR-126 level may suggest a novel therapeutic approach in breast
cancer treatment. However, an animal models study is needed to address and prove predictive ability of miR-126 on
breast cancer controlling.

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