Studying the Impact of Golgi Protein 73 Serving as a Candidate Biomarker in Early Diagnosis for Hepatocellular Carcinoma among Saudi Patients

Document Type: Research Articles

Authors

1 Health Sciences Research Center (HSCR), Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia.

2 Department of Biology, Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia.

3 Department of Medicine, Oncology Centre, King Khalid University Hospital, King Saud University, Kingdom Saudi Arabia.

4 Department of Molecular Biology, Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia.

5 Central Labe, King Saud University Riyadh, Kingdom Saudi Arabia.

6 Faculty of Medicine, EL-Azher University and Specialist Physician, Oncology Clinic-Medical Affaies, Tawam Hospital, AL Ain, UAE.

Abstract

Background: Due to the prevalence of Hepatocellular carcinoma (HCC) in Saudi Arabia, using new markers to
give best diagnostic performance than alpha-feto protein (AFP) are important in early diagnosis. The aim of this work
was to compare the significance between serum and mRNA Golgi glypican73 (GP-73) as newly identified diagnostic
and prognostic markers for HCC among Saudi patients. Materials and Methods: A total of 300 subjects were divided
into: 250 blood samples where 145 samples from HCC, 105 samples from chronic liver cirrhosis (CLC) and 50 normal
controls were investigated for serum GP73 (sGP73) by ELISA. GP-73 mRNA from peripheral blood mononuclear cells
was amplified by RT-PCR. The sensitivity and specificity of both techniques was compared. Results: Serum Golgi
glypican 73 was significantly higher in HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity
and specificity were 95% for sGP-73, 100% and 90% for Golgi glypican 73 mRNA. The combination of sensitivity
between AFP and sGP73 was 80% and 95% respectively. Conclusion: Both serum Golgi glypican-73 and GP-73Mrna
are good diagnostic biomarkers for early detection of HCC in Saudi patients. RT-PCR is more accurate and sensitive
(100%) than ELISA (95%) in detecting Golgi glypican 73.

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