The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma - Grade II, III and IV (NOS)

Document Type: Research Articles

Authors

1 Department of Pathology, Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER), Puducherry, India.

2 Department of Neurosurgery, Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER), Puducherry, India.

3 Department of Biostatistics, Jawaharlal Institute of Post Graduate Medical Education and Research (JIPMER), Puducherry, India.

Abstract

Background: Leptin, an adipocytokine functions via the leptin receptor, OB-Rb that contains an intact intracellular
domain and activates the JAK/STAT signalling cascade. It stimulates growth, migration and invasion of cancer cells in
vitro potentiating angiogenesis. Recently, the involvement of leptin in tumor progression is being explored. Gliomas
exhibit poor prognosis, low survival rates demanding for novel therapeutic regimens resulting in discovery of many
potential biomarkers and pharmaceutical targets. We analysed the potential role of leptin and OB-Rb in carcinogenesis
of malignant gliomas. Methods: Sixty fresh tissue samples of diffuse gliomas were collected after tumor excision. Real
time PCR, immunohistochemical (IHC) analysis and western blot analysis were carried out to assess the expression of
leptin and its receptor. Results: The present study demonstrates the expression of leptin and LepR and their involvement
in tumor progression. Of the 60 cases, 57 cases (95%) and 53 cases (88.3%) showed amplification for leptin and
OB-Rb respectively. The expression of these proteins were measured semi-quantitatively and correlated with degree of
malignancy (p<0.05). The bands were visualised on western blot. Conclusion: Leptin may be valued as a pharmaceutical
target and anti-leptin compounds could be developed as drugs in mono- or combined therapies for these tumors.

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