Comparison of Colposcopic Biopsy Results of Patients Who have Cytomorphological Normal but HPV 16-18 or Other High-Risk HPV Subtypes Positive

Objective: Current guidelines suggest annual co-test follow-up in non-16/18 high-risk HPV positive patients without abnormal cytologic findings. Considering the relatively high false-negative rates of cervical cytology, a follow-up period of one year might constitute an additional risk for development of cervical malignancies in these patients. The current study aims to evaluate colposcopic biopsy results of cytologically normal patients detected to carry high risk HPV by screening tests. Materials and Methods: A total of 160 patients with normal cervical cytology and high-risk HPV subtypes who underwent colposcopic examination were included in the study. Patients were divided into two groups according to their HPV results: HPV 16-18 and other types (non-16/18 high-risk subtypes). ECC, cervical biopsy, LEEP/conization results were analyzed for both groups. Histopathological results of the groups were compared in terms of ≤LSIL, HSIL and cervical cancer rates. Results: Cervical biopsy results in the “16-18” group were assessed as HSIL in 40 (48.2%) patients, LSIL in 6 patients (7.2%) and normal in 37 (44.6%) patients. ECC results revealed HSIL in 9 (10.8%) patients and LSIL in 1 (1.2%) patient. Nineteen out of 42 patients who underwent LEEP/conization had HSIL (surgical margin positivity was reported in 4 cases), while 3 patients had LSIL. None of the cases had cervical carcinoma. Conclusion: The present study detected that 15.6% of women infected with non-16/18 high-risk HPV subgroups developed ≥HSIL lesions. Although this rate seems lower than HPV 16-18 group, it is still too high to be overlooked. In conclusion, we suggest further clinical trials with larger number of patients to be conducted on this topic.


Introduction
National Cancer Institute, Centers for Disease Control and Prevention, Jemal et al., (2013) and Viens et al., (2016) report that HPV infection is associated with nearly all (more than 90%) of cervical cancers (National Cancer Institute, 2011; Centers for Disease Control and Prevention, 2012). HPV 16 alone is responsible from half of all cervical cancers, while HPV 18 is responsible from 20% (National Cancer Institute, 2011). Fifteen HPV subtypes (HPV 16,18,31,33,35,39,45,51,52,56,58,59,68,73,82) are defined as high-risk (hrHPV) for the development of cervical cancer by Centers for Disease Control and Prevention, 2011;Jemal et al., 2013) The ASCCP guidelines recommended immediate colposcopy in HPV 16/18 positive cases, regardless of cytology results, while co-testing after 1 year is suggested in the presence of other hrHPV subtypes with a negative cytology (ASCCP Guidelines, 2013). Two studies conducted in Turkey reported 20% of women with normal cervical cytology to be HPV-DNA positive and 32.5% of

Comparison of Colposcopic Biopsy Results of Patients Who have Cytomorphological Normal but HPV 16-18 or Other High-Risk HPV Subtypes Positive
Hüseyin Aydoğmuş 1 *, Serpil Aydoğmuş 2 hrHPV positive women to have normal cervical cytology (Dursun at al., 2009;Beyazit at al., 2018). Various studies have reported the false negative rate of cervical cytology to be between 15% and 63% (Lönnberg at al., 2010;Banna at al., 2014). Additionally, patients might interrupt their annual follow-ups for various reasons.
The aim of this study is to compare colposcopic biopsy results of HPV 16/18 positive patients and patients carrying at least one of the hrHPV subtypes both with normal cervical cytology.

Materials and Methods
This retrospective cohort study has been conducted between January 2015 and December 2017 in a tertiary center located in western Turkey at the Clinic of Obstetrics and Gynecology, Izmir Katip Çelebi University Ataturk Training and Research Hospital. A total of 167 women referred to our hospital due to HPV DNA positivity from primary screening centers were included in the study. The study protocol was approved by the İzmir Katip Çelebi University Ethics Committee (2017-308). Patients who did not accept colposcopy and biopsy, or had previous history of cervical dysplasia were excluded from the study. Informed consent was obtained from all participants and the study was in agreement with the Declaration of Helsinki for Medical Research Involving Human Subjects. Cervical cytologic evaluation was performed by conventional or liquid-based cytology. Abnormal histopathologic findings were classified as low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC). Hybrid Capture2 (Qiagen) kit for HPV-DNA scans and CLART kit (Genomica) or Cobas ® HPV kit (Roche) for HPV genotyping were utilized. A total of 160 patients who were examined for at least one of the high-risk DNA subtypes and were found to have no abnormal findings on cervical cytology were included in the final statistical evaluation. All patients were evaluated by a gynecologist experienced in colposcopy (HA). Patients were divided into two groups according to HPV results as HPV 16/18 positive (group 1, HPV 16/18) and as non-16/18 hrHPV subtypes (group II, OTHERS). Endocervical curettage (ECC), cervical biopsy, LEEP/conization results of both groups were analyzed. Histopathologic results of the groups were compared in terms of ≤LSIL, HSIL and cervical squamous cancer rates. Eighty-three patients in the HPV 16/18 group and 77 patients in the 'OTHERS' group were evaluated.
The most common subtype in the "Others" group was found to be HPV 52 (15.5%). Cervical biopsy results were assessed as HSIL in 12 (15.6%) patients, LSIL in 6 (7.8%) patients and normal in 59 (76.6%) patients. ECC results revealed 1 HSIL (1.3%) and 1 LSIL. Eight out of 12 patients who underwent LEEP/conization had HSIL (surgical margin positivity was reported in a single case), while none had LSIL nor cervical carcinoma.
Evaluation of the groups regarding histopathological findings showed that 48.2% of the cases in the "16-18" group had ≥HSIL lesions while only 15.6% of the "others" group had ≥HSIL lesions (p˂0.01). Presence of HPV 16-18 resulted in 5-fold increased risk of developing ≥ HSIL lesions compared to other high-risk HPV subtypes (OR: 5.03% 95% CI 2.3-10.6).