Evaluation of Ultra-Microscopic Changes and Proliferation of Apoptotic Glioblastoma Multiforme Cells Induced by Velogenic Strain of Newcastle Disease Virus AF2240

Document Type : Research Articles


1 Faculty of Dentistry, MAHSA University, Malaysia.

2 Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Malaysia.

3 Faculty of Veterinary Medicine, University Putra Malaysia, Malaysia.

4 Institute of Bioscience, University Putra Malaysia, Malaysia.

5 Faculty of Agriculture and Biotechnology, University Sultan Zainalb Abidin, Malaysia.

6 Institute of Agrobiotechnology, University Sultan Zainalb Abidin, Malaysia.

7 Natural Medicine Research Centre, University Islam Malaysia, Malaysia.


Aim: Newcastle disease virus (NDV) is a member of genus Avulavirus within the family Paramyxoviridae. Interest
of using NDV as an anticancer agent has arisen from its ability to kill tumor cells with limited toxicity to normal cells.
Methods: In this investigation, the proliferation of brain tumor cell line, glioblastoma multiform (DBTRG.05MG)
induced by NDV strain AF2240 was evaluated in-vitro, by using MTT proliferation assay. Furthermore, Cytological
observations were studied using fluorescence microscopy and transmission electron microscopy, DNA laddering in
agarose gel electrophoresis assay used to detect the mode of cell death and analysis of the cellular DNA content by
flowcytometery. Results: MTT proliferation assay, Cytological observations using fluorescence microscopy and
transmission electron microscopy show the anti-proliferation effect and apoptogenic features of NDV on DBTRG.05MG.
Furthermore, analysis of the cellular DNA content showed that there was a loss of treated cells in all cell cycle phases
(G1, S and G2/M) accompanied with increasing in sub-G1 region (apoptosis peak). Conclusion: It could be concluded
that NDV strain AF2240 is a potent antitumor agent that induce apoptosis and its cytotoxicity increasing while increasing
of time and virus titer.


Main Subjects