ТР53 Codon 72 Polymorphism and Human Papilloma Virus-Associated Cervical Cancer in Kyrgyz Women

Document Type: Research Articles

Authors

1 Institute of Molecular Biology and Medicine, Bishkek, Kazakhstan.

2 School of Public Health, Al-Farabi Kazakh National University, Almaty, Kazakhstan.

3 National Center of Oncology and Hematology, Bishkek, Kazakhstan.

Abstract

Background: The aim of this study was to ascertain the magnitude of association of gene ТР53 Arg72Pro polymorphic
marker with cervical cancer (CC) in Kyrgyz women. Methods: We identified and included 205 women of Kyrgyz
ethnicity for this case-control study, of whom N=103 were women (mean age 53.5 ± 10.0 years) with histologically
confirmed CC and N=102 controls (mean age 46.5 ± 8.5 years). We detected human papilloma virus (HPV) DNA types
16 and 18 using polymerase chain reaction (PCR) with hybridization/fluorescent detection. Genotypes of ТР53 gene
Arg72Pro polymorphism were identified using PCR-RFLP assay. Results: Eighty-eight percent (90/103) women with
CC had HPV, of whom 43.4% (39/90) had HPV type 16, 24.4% (22/90) had HPV type 18, whereas 32.2% (29/90)
carried both types. The univariate analysis of allele and genotype distribution of Arg72Pro polymorphic marker of ТР53
gene showed no difference between CC and control groups (χ2=1.24, р=0.54). However, when CC cases associated with
HPV were tested against controls, Arg72 allele and Arg72Arg genotype prevalence were greater compared to controls
(χ²=7.25; р=0.027 for genotypes and χ²=6.83; р=0.009 for alleles). In HPV-positive women, Arg72Arg genotype of
ТР53 gene was associated with a 1.85-fold increase in the likelihood of CC (OR=1.85 [95% confidence interval (CI)
1.03-3.32]), whereas Arg72 allele increased this likelihood 1.94-fold (OR=1.94 [95% CI 1.20-3.15]). Conclusions:
Arg72Arg genotype and Arg72 allele of ТР53 gene in Kyrgyz women increase the risk of HPV-associated CC.

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