Survivin Promoter Polymorphism (-31 C/G): A Genetic Risk Factor for Oral Cancer

Document Type: Research Articles

Authors

1 Department of Pathology, Ziauddin University Clifton campus, Karachi, Pakistan.

2 Department of Molecular Genetic, Ziauddin University North Nazimabad campus, Karachi, Pakistan.

3 Department of Community medicine, Ziauddin University Clifton campus, Karachi, Pakistan.

Abstract

Background: The polymorphism of survivin gene at its promoter region is one of the risk factors for OSCC . This
polymorphism involves substitution of G for C (9904341), and it is present at the cell cycle dependent elements and
cell cycle homology region repressor binding motif of promoter. This study aimed to find the association between
survivin -31C/G polymorphism and prevalence of OSCC in a subset of Pakistani population. Methodology: This
case-control study was conducted on 47 cases with and 101 healthy individuals with no family history of cancer. We used
polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) protocols. Results: The most
common site of oral cancer in our research was the buccal mucosa followed by tongue and the least one was the labial
mucosa. The histological tumor type of all 47 cases was squamous cell type. In our research, stage II had the highest
prevalence, accounting for 34% of patients, while the prevalence of stage I was 31% in the case group. The prevalence
of stage III and IV was 25% and 8%, respectively. The numbers of moderately and poorly differentiated tumors were
equal. We found a significant association between the CC genotype of survivin and OSCC prevalence (OR was 9.395
at 95% CI: 1.0202-86.5251, p-value= 0.04). The GG genotype also showed significant P value (OR: 0.4709 with 95%
CI: 0.2323- 0.9546 at a P VALUE of 0.0367). while no significant P value was noted for CG genotype (OR: 1.4317 with
95% CI: 0.7513 -2.8658, p- value= 0.31). Conclusion: Survivin -31G/C polymorphism was strongly associated with
OSCC prevalence. The C allele was more common in case group as compared to healthy individuals living in Pakistan.

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