Document Type: Research Articles
Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, India.
Department of Pathology, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, India.
Department of Surgical Oncology, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, India.
Department of Radiation Oncology, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, India.
Background: The aim of this study was to evaluate microvessel density (MVD) by expression of CD31 and
CLEC14A in core biopsies from previously untreated patients with locally advanced breast cancer (LABC) and assess
its prognostic significance. Methods: MVD was evaluated in core needle biopsies (n = 92), collected prior to any
treatment, from patients who were diagnosed with locally advanced breast cancer (LABC). Immunohistochemistry for
expression of CD31 and CLEC14A were performed on these tumours. The median duration of follow-up was 9.3 years.
The effect of prognostic factors on disease free survival (DFS) and overall survival (OS) was assessed using a Log rank
test and Cox regression model. Results: The clinical factors such as age, clinical nodal stage, stage and pathological
nodal status were found to be significant in predicting overall survival by multivariate analysis (P<0.05). Out of 92, 52
tumours had blood vessels expressing CD31, whereas in the remainder, there was no expression. The mean and median
MVD of CD31 in 92 tumours was 38 and 5.5 respectively, and it was not a significant factor for predicting disease free
survival or overall survival. When we considered the tumours (n=52) which expressed CD31, patients who had very
high MVD (>100), had inferior progression free survival and overall survival (P=0.5). There was no expression of
CLEC14A in any of the core needle biopsies whereas it was expressed in specimens from mastectomy from the same
patient. Conclusion: This is the first report of MVD in LABC prior to any treatment. The results suggest angiogenesis
could be a prognostic factor in LABC.