Ionizing Radiation-inducible microRNA-21 Induces Angiogenesis by Directly Targeting PTEN

Document Type: Research Articles

Authors

Department of Radiotherapy, Affiliated Hospital of Qingdao University, Qingdao, China.

Abstract

Background: Previous experimental studies have established that MicroRNAs (miRNAs) can function as oncogenes
or tumor suppressors in the regulation of tumor biology or pathology. However, the effects of ionizing radiation (IR)
on the expression levels of cellular miRNAs and their further effects on the biological behavior of tumor cells require
further investigation. Methods: We determined the proliferation, migration and tube formation of HUVEC cells after
ionizing radiation (control, 0h and 24h), and the changes of miR-21, VEFG and HIF-1α levels after ionizing radiation were
measured by Western blot (WB). The effects of miR-21 mimics and inhibitors on the protein and mRNA expression of
PTEN were determined by RT-PCT and WB. Two independent luciferase reporter plasmids were constructed to detect
changes in PTEN protein expression. Results: We found that both IR and miR-21 increase proliferation, migration and
tube formation of HUVEC cells. Ionizing radiation directly targets the inhibition of PTEN expression by causing an
increase in miR-21 expression, and induces the accumulation of VEGF and HIF-1α expression in cells by the PI3K / AKT
signaling pathway. Simultaneous induction of ectopic expression of PTEN can rescue radiation-induced proliferation,
migration and tube formation in tumor cells. Conclusion: miR-21 promotes tumor cell proliferation and migration by
targeting inhibition of PTEN expression, which may become a potential target for tumor therapy in the future.

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