Document Type: Research Articles
Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen,Thailand.
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand.
Background: The use of targeted specific genes in therapeutic and treatment decisions has been considered for
lung cancer. The epidermal growth factor receptor (EGFR) gene, which is over expressed in non-small cell lung cancer
(NSCLC), was considered as one of the targeted specific genes. EGFR mutations in exons 18–21, which encode a
portion of the EGFR kinase domain, were found in NSCLC patients and were associated with the response of EGFRtyrosine
kinase inhibitors (EGFR-TKIs). Therefore, a molecular technique for EGFR mutation detection has important
benefits for therapy in NSCLC patients. This study aims to determine the EGFR mutations in patients with NSCLC
using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) in exons 18-21. Methods:
DNA samples were extracted from formalin fixed paraffin embedded tissues of NSCLC patients who attended hospital.
The extracted DNA was used as a template for the EGFR gene amplification. Results: Occurrence of EGFR mutations
were found in 29 out of 50 cases (58%).The frequency of EGFR mutations by first PCR at exon 18, 19, 20 and 21
were 6 (12%), 19 (38%) 20 (40%) and at 21 (42%), respectively. By PCR-SSCP, the frequencies of EGFR mutations
at exon 18, 19, 20 and 21 were 3(6%), 18(36%), 23(46%) and 13(26%), respectively. All of the mutations found were
in agreement with DNA sequencings. Conclusion: The high frequency of EGFR mutations in NSCLC suggests that
PCR-SSCP is a efficient screening method and useful for treatment plan.