Document Type : Short Communications
Department of Haematology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan.
Department of Hematology, School of Medical Sciences, University Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.
Department of Haematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
Hematology and Blood Banking Unit, Medical Laboratory Technology Department, Jazan University, Jazan, KSA.
Background: Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and antiangiogenicfunctions
and may have both tumor-promoting and -inhibiting properties. We examined the association
between a single nucleotide polymorphism (SNP) in IL-10 -1082G/A (rs1800896) in Sudanese acute myeloid leukemia
(AML) patients and to assess the association between polymorphisms in IL-10 -1082G/A (rs1800896) and the
hematological profile in Sudanese patients with AML. Methods: A total of 30 patients with acute myeloid leukemia
and 30 control subjects were enrolled in this study. Blood samples were collected from all patients in EDTA containing
tubes. Genomic DNA was extracted from all blood samples using salting out method. The genotypic variants of
IL-10 (-1082G/A) polymorphism were detected by allele specific-PCR. Results: We found that (36.7%) of patients have
homogenous GG genotype, (43.3%) have heterogeneous GA genotype and (20.0%) have AA genotype. GA genotype
was significantly associated with higher risk of AML compared with the homozygous Genotypes (GG and AA), there is
no association between IL-10 (-1082G/A) polymorphism and AML sub-type, gender, age group, mean of hematological
parameters. Conclusion: Our study concluded that GA genotype of IL-10 -1082G/A (rs1800896) polymorphism is a
risk factor for AML and G allele is insignificantly higher than A allele in AML patient. No association between IL-10
(-1082G/A) polymorphism and AML sub-type, gender, age group, mean of hematological parameters.