Investigating the TLR9 mRNA Expression Level in Different Histological Types of Colorectal Polyps

Document Type: Research Articles

Authors

1 Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in
inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer
cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9
in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC
initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The
mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was
evaluated by 2-ΔΔct method. There was a significant relationship between the lower expression of TLR9 gene in the polyp
cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in
adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies.

Keywords

Main Subjects