Genetic Variants Related to Cell Cycle and Stability of Telomere in Patients with Glioma

Document Type: Research Articles

Authors

1 Department of Biochemistry and Molecular Biology, Faculty of Medicine , São José do Rio Preto, São Paulo, Brazil.

2 Departament Molecular Biology on the Sao Paulo State University-“Júlio de Mesquita Filho” Campus- UNESP/IBILCE, Brazil.

3 Departament of Patology of the Hospital de Base University Hospital of the Medical School of São José do Rio Preto - HB/FAMERP, Brazil.

4 Department of Physical Education of the Sao Paulo State University- “Júlio de Mesquita Filho” Campus- UNESP/Campos de Bauru, Brazil.

5 Department of Neurology of Santa Casa of São Paulo Medical Science College, Brazil.

6 Departament of Neurology of the Medical School of São José do Rio Preto – FAMERP, Brazil.

Abstract

Background: Glioma, most common primary malignant brain tumor in adults, is highly aggressive and associated
with a poor prognosis. Evaluate the association of polymorphisms related of to the cell cycle, integrity and DNA repair
with gliomas, as well as lifestyle habits, comorbidities, survival and response to treatment. Methods: Were studied
303 individuals distributed into: Study Group - 100 patients with gliomas, regardless of the degree of malignancy, and
Control Group - 203 individuals without clinical signs of the disease. These polymorphisms were genotyped by TaqMan®
SNP Genotyping Assay. Significance level was set at 5%. Results: Smoking, alcohol consumption, systemic arterial
hypertension (SAH) and diabetes mellitus (DM) prevailed in patients, compared to controls (P=0.0088, P=0.0001,
P=0.0001, P=0.0011, respectively). In the logistic regression analysis, alcohol consumption and SAH were identified
as independent risk factors for gliomas (P=0.0001, P=0.0027, respectively). Patients with low-grade gliomas showed
survival in one year (92.0±6.8%), compared to patients with high-grade gliomas (24.0±5.3; P=0.011). Conclusion:
Polymorphisms involved in cell cycle, telomere protection and stability and DNA repair are not associated with gliomas.
On the other hand, alcohol consumption and SAH stand out as independent risk factors for the disease. Low-grade
gliomas, response to treatment and the combination of chemotherapy with Temozolomide and radiation therapy show
increased survival of patients.

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