Molecular Screening of Nasopharyngeal Carcinoma: Detection of LMP-1, LMP-2 Gene Expression in Vietnamese Nasopharyngeal Swab Samples

Document Type: Research Articles

Authors

1 Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.

2 Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam.

3 Cho Ray Hospital, HCMC, Vietnam.

Abstract

Objective: This study aimed to characterize the expression of LMP-1, LMP-2 in clinical swab samples in order
to find out the potential molecular based biomarker for NPC diagnosis and screening, which could offer a chance in
development of rapid method for NPC diagnosis in Vietnamese population. Materials and Methods: A total of 93
nasopharyngeal carcinoma swab samples and 100 healthy nasopharyngeal swab samples were collected to evaluate
LMP-1, LMP-2 expression by Real-time reversed PCR. Results: we figured out the significant association between
the expression of LMP-1 (counting for 48.39%), LMP-2 (counting for 39.78%) and NPC. No LMP-1 expression was
observed, and only 1 of 100 specimens was detected with LMP-2 positive in healthy samples. In the combination of
LMP-1 (+) and/or LMP-2 (+), the frequency of positive was 53.76%, greater than each gene expression. Additionally,
sensitivity, specificity, positive predictive value, negative predictive value of assay were 99.00%, 98.04%, 69.72%,
and 77.02%, respectively. Additionally, the LMP-2 expression level was 5.50 times higher in NPC samples than
non-cancerous samples. Conclusion: Our results indicated the molecular invasive method based on the expression of
LMP-1, LMP-2 in swab samples would be a promising supplement in NPC diagnosis, screening in the near future in
Vietnam.

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