Document Type : Research Articles
Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.
Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam.
Cho Ray Hospital, HCMC, Vietnam.
Objective: This study aimed to characterize the expression of LMP-1, LMP-2 in clinical swab samples in order
to find out the potential molecular based biomarker for NPC diagnosis and screening, which could offer a chance in
development of rapid method for NPC diagnosis in Vietnamese population. Materials and Methods: A total of 93
nasopharyngeal carcinoma swab samples and 100 healthy nasopharyngeal swab samples were collected to evaluate
LMP-1, LMP-2 expression by Real-time reversed PCR. Results: we figured out the significant association between
the expression of LMP-1 (counting for 48.39%), LMP-2 (counting for 39.78%) and NPC. No LMP-1 expression was
observed, and only 1 of 100 specimens was detected with LMP-2 positive in healthy samples. In the combination of
LMP-1 (+) and/or LMP-2 (+), the frequency of positive was 53.76%, greater than each gene expression. Additionally,
sensitivity, specificity, positive predictive value, negative predictive value of assay were 99.00%, 98.04%, 69.72%,
and 77.02%, respectively. Additionally, the LMP-2 expression level was 5.50 times higher in NPC samples than
non-cancerous samples. Conclusion: Our results indicated the molecular invasive method based on the expression of
LMP-1, LMP-2 in swab samples would be a promising supplement in NPC diagnosis, screening in the near future in