Document Type: Research Articles
Laboratory of Molecular Biology of Viruses, Biological Sciences Institute, University of Pernambuco, Brazil.
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Biological Sciences Center, Federal University of Pernambuco, Recife, Brazil.
Background: The objective of this study was to analyze the gene expression profile of the proinflammatory
interleukins, (IL-1β and IL-18) in patients with premalignant lesions and cervical cancer. Methods: Total IL-1β and
IL-18 mRNA was quantified by qPCR to obtain the expression data in cervical tissues. A total of 74 cervical biopsies
were obtained from women undergoing a colposcopy. The samples were divided into: normal (19), low level lesions
(LSIL) or NIC I (17), high level lesions (HSIL) or CIN II and CIN III (29) and cancer (9). The normal cervical tissue
samples were included as controls. The OR and 95% CI were calculated for the determination of the risk of progression
between each type of lesion and cancer using logistic regression. Results: The results showed that an increase in the
risk of progression of pre-neoplastic lesions to cancer was between 2.5 and 2.08 times higher in women with lower
IL-1β and IL-18 expression, respectively. Conclusions: This study provided evidence that IL-1β and IL-18 are potential
biomarkers that can be explored in further studies for monitoring the evolution of pre-neoplastic lesions and avoiding
overtreatment or undertreatment of the patients.