PIK3CA and PTEN Genes Expressions in Breast Cancer

Document Type: Research Articles

Authors

1 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.

2 Department of Clinical Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

3 Surgical Oncology Unit, Department of General Surgery, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

Abstract

Background: The phosphatidylinositol-3 kinase (PI3K) intracellular signaling pathway plays an important
role in breast cancer. The current study aimed to evaluate the expressions of two main regulators of PI3K pathway;
phosphatidylinositol-3- kinase catalytic subunit alpha as activator (PIK3CA), and phosphatase and tensin-homolog
as inhibitor (PTEN), in breast carcinoma tissue, and compare with their expressions in adjacent normal breast tissue.
Methods: A total of fifty female patients with breast carcinoma from surgical oncology unit of Alexandria-Main
University Hospital were included in this study. The Quantitative Real Time PCR was used to quantify expressions of
PIK3CA and PTEN. Results: PIK3CA mRNA expression was significantly increased in breast cancer tissues compared
to normal breast tissues (P<0.001, Z=5.700), also PTEN mRNA expression was significantly higher in breast carcinoma
tissue compared to normal breast tissue (P<0.001, Z=5.362). Conclusion: Increased the expressions of PIK3CA and
PTEN mRNA in breast cancer tissue compared to normal breast tissue.

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