Document Type: Research Articles
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Department of Clinical Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Surgical Oncology Unit, Department of General Surgery, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Background: The phosphatidylinositol-3 kinase (PI3K) intracellular signaling pathway plays an important
role in breast cancer. The current study aimed to evaluate the expressions of two main regulators of PI3K pathway;
phosphatidylinositol-3- kinase catalytic subunit alpha as activator (PIK3CA), and phosphatase and tensin-homolog
as inhibitor (PTEN), in breast carcinoma tissue, and compare with their expressions in adjacent normal breast tissue.
Methods: A total of fifty female patients with breast carcinoma from surgical oncology unit of Alexandria-Main
University Hospital were included in this study. The Quantitative Real Time PCR was used to quantify expressions of
PIK3CA and PTEN. Results: PIK3CA mRNA expression was significantly increased in breast cancer tissues compared
to normal breast tissues (P<0.001, Z=5.700), also PTEN mRNA expression was significantly higher in breast carcinoma
tissue compared to normal breast tissue (P<0.001, Z=5.362). Conclusion: Increased the expressions of PIK3CA and
PTEN mRNA in breast cancer tissue compared to normal breast tissue.