Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients

Document Type: Research Articles

Authors

1 Oncology Group, Gastroenterology Division, Medicine Department, Universidade Federal de Sao Paulo, São Paulo, Brazil.

2 Gastroenterology Division and Endoscopist, Universidade Federal de Sao Paulo, São Paulo, Brazil.

3 Surgery Department, Universidade Federal de Sao Paulo, São Paulo, Brazil.

4 Randox Laboratories Ltd., Crumlin, Co. Antrim, United Kingdom.

Abstract

Background: Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already
described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and
significant improvement at public health. Aim: to analyze stool DNA by human DNA quantify and microarray
methods as alternatives to CRC screening. Method: Three methods were analyzed in stool samples: Human DNA
Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. Results: KBP array mutations were presented in
60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for
human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity
and RanplexCRC Array method had 78% sensitivity and 100% specificity. Conclusion: Microarray methods showed
promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy
and applicability by clinical routine.

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