Anti-Proliferative Effects of Ziziphus spina-christi and Phlomis Russeliana Leaf Extracts on HEK293 and MCF-7 Cell Lines and Evaluation of Bax and Bcl-2 Genes Expression Level in MCF-7 Cells

Document Type: Research Articles

Authors

1 Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

2 Department of Physiology, Faculty of basic Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran.

3 Department of Microbiology, Faculty of bio Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.

4 Department of Biotechnology, Factualy of BioScineces, North Tehran Branch, Islamic Azad University, Tehran, Iran.

Abstract

To investigate the effects of Phlomis russeliana and Ziziphus spina-christi leaf extracts on apoptosis in breast cancer MCF-7 cells.  Cell lines were divided into control group and groups exposed to 0.001, 0.01, 0.1, 1 mg/ml of Ziziphus spina-christi and Phlomis russeliana leaf extracts. Cell viability was quantified by MTT assay. The expression of Bax and Bcl-2 genes was evaluated by Real time PCR analysis. Statistical analysis was performed using ANOVAHEK239 cells viability significantly increased in groups exposed to 0.001, 0.01 and 0.1 mg/ml of Z.christi leaf extract and decreased in group exposed to 10 mg/ml of P.russeliana leaf extract. MCF-7 cells viability significantly decreased in groups exposed to 0.001 ,0.01, 0.1, 1 and 10 mg/ml of Z.christi leaf extract and increased in groups exposed to 0.001 and 0.01 mg/ml of P.russeliana leaf extract. Exposure of MCF-7 cells to 1 and 10 mg/ml of P.russeliana leaf extract also led to significant decrease in cell viability. The cytotoxic effect of Z.christi was higher than P.russeliana leaf extracts on MCF7 cells.  1 mg/ml of Z.christi leaf extracts also significantly increased the expression level of both Bax and Bcl-2 genes in MCF7 cells. Bcl-2 gene expression significantly increased in group exposed to 10 mg/ml of P.ruseliana leaf extract.Despite P.russeliana leaf extract, lower Z.christi leaf extract concentrations inhibited MCF-7 cells proliferation. Ziziphus spina-christi and phlomis russeliana leaf extracts mechanism of action is occurred through Bax-independent apoptotic pathway on MCF-7 cells.

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Articles in Press, Accepted Manuscript
Available Online from 29 June 2020
  • Receive Date: 05 February 2020
  • Revise Date: 29 June 2020
  • Accept Date: 29 June 2020