Document Type: Research Articles
Department of Biophysics, Malayer University, Malayer, Iran.
Department of Biomedical Engineering, Faculty of Health, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Medical physics and physiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
This research aimed to assess the impact of static magnetic field (SMF) on apoptosis rate and cell cycle progression in the presence of Aloe vera Crude Extract (ACE) in normal (Huo2) and cancer cells (HeLa). The specimens were split into one untreated group (control) and two experimental groups, including treatment with ACE (Alo) and compound treatment with SMF and ACE (Alo+SMF). MTT assay determined the IC50 value, and flow cytometry was employed to evaluate cell cycle distribution and apoptosis rates. Statistical analysis was carried out through a two-way ANOVA followed by Tukey’s post hoc test. Our results showed that combination treatment with SMF (10 mT) and ACE (Alo+SMF) significantly inhibited the cell proliferation. This increased the cell number in G2/M stage and early apoptosis in cancer cells compared to ACE treated cells after 24 and 48h but reduced the number of Huo2 cells in G2/M phase and early apoptosis after 24h. The effect of AEC on HeLa cells was intensified with increasing the SMF exposure time, such that the early apoptosis rate in Alo+SMF group had an approximate 4-fold increase compared to Alo group. This research proposes that the combination treatment accelerates the apoptosis induction of HeLa cell. During the interphase, there were significant differences between the cancer and healthy cells concerning the cell cycle. Moreover, exposure time may play an important role in the impact SMF on both healthy and cancer cells in the presence of AEC.