Impact of Bone Marrow Natural Killer Cells (NK); Soluble TNF-α and IL-32 Levels in Myelodysplastic Syndrome Patients

Aref, Salah; Khaled, Nada; Al Gilany, Abdel Hady; Ayed, Mohamed; Abouzeid, Tarek; Attia, Doaa

doi:10.31557/APJCP.2020.21.10.2949

Impact of Bone Marrow Natural Killer Cells (NK); Soluble TNF-α and IL-32 Levels in Myelodysplastic Syndrome Patients

Document Type : Research Articles

Authors

¹ Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.

² Public Health and Biostatistics Department, Mansoura University, Egypt.

³ Hematology Unit, Mansoura University Oncology Center, Mansoura University, Egypt.

10.31557/APJCP.2020.21.10.2949

Abstract

Background: Myelodysplastic syndromes (MDS) are complex clonal hemopoietic progenitor cell disorders that result from the evolution of aberrant clones which lead to leukemia. Disorders of the immune system serve important functions in the pathophysiology and progression of this disorder. This study aimed to assess the bone marrow natural killer cells percentage as well as soluble TNF-α and sIL-32 concentration levels in MDS patients. Methods: Bone marrow samples were obtained from 34 MDS; 12 MDS-AML and 10 controls. The percentage of total NK cells and mature NK cells were determined by flowcytometry. Bone Marrow soluble TNF-α and sIL-32 concentration levels were measured by ELISA. Results: The percentage of total NK and mature NK cells were significantly lower in MDS patients as compared to controls (p

Keywords