Department of Endoscopy Center, Institute of Shanxi Traditional Chinese Medicine, Hospital of Shanxi Traditional Chinese Medicine, Taiyuan, Shanxi, China.
Background: Rosmarinic acid (RA) is a natural phenolic compound that acts as a Fyn inhibitor by 53 homology modeling of the human Fyn structure. Therefore, the apoptosis mechanism related to NF-κB signaling pathway induced by RA in HepG2 was investigated. Methods: The cell growth, apoptosis, and proliferation of HepG2 regulated by various concentrations of RA were studied. The proteins expression of MMP-2, MMP-9, PI3K, AKT, NF-κB, and apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3 were detected. Results: RA significantly reduced proliferation rates, inhibited migration and invasion, and decreased the expressions of invasion-related factors, such as matrix metalloproteinase (MMP)-2 and MMP-9. TUNEL staining revealed that RA resulted in a dose-dependent increase of HepG2 cell apoptosis. In line with this finding, the expression of apoptosis suppressor protein Bcl-2 was downregulated and that of the pro-apoptotic proteins Bax and cleaved caspase-3 was increased. In addition, we found that the phosphatidylinositol 3-kinase (PI3K)/Akt/nuclear factor kappa B (NF-κB) signaling pathway was involved in RA-mediated inhibition of HepG2 cell metastasis. Conclusion: Our study identified that RA as a drug candidate for the treatment of HCC.
An, Y., Zhao, J., Zhang, Y., Wu, W., Hu, J., Hao, H., Qiao, Y., Tao, Y., & An, L. (2021). Rosmarinic Acid Induces Proliferation Suppression of Hepatoma Cells Associated with NF-κB Signaling Pathway. Asian Pacific Journal of Cancer Prevention, 22(5), 1623-1631. doi: DOI:10.31557/APJCP.2021.22.5.1623
MLA
Yanjun An; Jiandong Zhao; Yourui Zhang; Wen Wu; Jiangtao Hu; Haosen Hao; Yu Qiao; Ying Tao; Liping An. "Rosmarinic Acid Induces Proliferation Suppression of Hepatoma Cells Associated with NF-κB Signaling Pathway". Asian Pacific Journal of Cancer Prevention, 22, 5, 2021, 1623-1631. doi: DOI:10.31557/APJCP.2021.22.5.1623
HARVARD
An, Y., Zhao, J., Zhang, Y., Wu, W., Hu, J., Hao, H., Qiao, Y., Tao, Y., An, L. (2021). 'Rosmarinic Acid Induces Proliferation Suppression of Hepatoma Cells Associated with NF-κB Signaling Pathway', Asian Pacific Journal of Cancer Prevention, 22(5), pp. 1623-1631. doi: DOI:10.31557/APJCP.2021.22.5.1623
VANCOUVER
An, Y., Zhao, J., Zhang, Y., Wu, W., Hu, J., Hao, H., Qiao, Y., Tao, Y., An, L. Rosmarinic Acid Induces Proliferation Suppression of Hepatoma Cells Associated with NF-κB Signaling Pathway. Asian Pacific Journal of Cancer Prevention, 2021; 22(5): 1623-1631. doi: DOI:10.31557/APJCP.2021.22.5.1623
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