Objective: Circulating cell-free mitochondrial DNA (cf-MtDNA) has been reported in patients with chronic obstructive pulmonary disease (COPD) and lung cancers. However, inter-relationships among the three biological events have not been well-characterized. Therefore, our investigation was conducted to better understand the role of cf-MtDNA on pathogenesis of the two diseases. Methods: Plasma samples were collected from 64 non-small cell lung cancer (NSCLC) patients (before therapy), 45 patients with COPD and 62 healthy individuals. cf-MtDNA copy numbers were detected using quantitative real-time polymerase chain reaction (qRT-PCR) and cytokines were determined using a human ELISA kit. Results: Our data indicate that smoking statuses of the patients and controls were significantly associated with increased cf-MtDNA in plasma samples. Furthermore, NSCLC patients had significantly higher cf-MtDNA copy numbers than COPD patients (p < 0.03) and normal controls (p < 0.02), together with elevated proinflammatory cytokines over the controls (p < 0.05). Our study shows that the copy numbers for the NSCLC patients were positively associated with their subsequent metastasis but inversely associated with their overall survival. Conclusion: Our study indicates certain lung injury (e.g., from cigarette smoking) was responsible for the release of cf-MtDNA and proinflammatory cytokines into plasmas among our patients and controls. The increase in cf-MtDNA copy numbers was significantly associated with the development of both COPD and NSCLC, with increase in interleukin 6, and from our 5-year follow-up, with poor prognosis among the NSCLC patients. Therefore, with further validation, cf-MtDNA can be considered for use as diagnostic and prognostic biomarkers for NSCLC.
Bulgakova, O., Kausbekova, A., Kussainova, A., Kalibekov, N., Serikbaiuly, D., & Bersimbaev, R. (2021). Involvement of Circulating Cell-Free Mitochondrial DNA and Proinflammatory Cytokines in Pathogenesis of Chronic Obstructive Pulmonary Disease and Lung Cancer. Asian Pacific Journal of Cancer Prevention, 22(6), 1927-1933. doi: 10.31557/APJCP.2021.22.6.1927
MLA
Olga Bulgakova; Asemgul Kausbekova; Assiya Kussainova; Nurtas Kalibekov; Dulat Serikbaiuly; Rakhmetkazhi Bersimbaev. "Involvement of Circulating Cell-Free Mitochondrial DNA and Proinflammatory Cytokines in Pathogenesis of Chronic Obstructive Pulmonary Disease and Lung Cancer". Asian Pacific Journal of Cancer Prevention, 22, 6, 2021, 1927-1933. doi: 10.31557/APJCP.2021.22.6.1927
HARVARD
Bulgakova, O., Kausbekova, A., Kussainova, A., Kalibekov, N., Serikbaiuly, D., Bersimbaev, R. (2021). 'Involvement of Circulating Cell-Free Mitochondrial DNA and Proinflammatory Cytokines in Pathogenesis of Chronic Obstructive Pulmonary Disease and Lung Cancer', Asian Pacific Journal of Cancer Prevention, 22(6), pp. 1927-1933. doi: 10.31557/APJCP.2021.22.6.1927
VANCOUVER
Bulgakova, O., Kausbekova, A., Kussainova, A., Kalibekov, N., Serikbaiuly, D., Bersimbaev, R. Involvement of Circulating Cell-Free Mitochondrial DNA and Proinflammatory Cytokines in Pathogenesis of Chronic Obstructive Pulmonary Disease and Lung Cancer. Asian Pacific Journal of Cancer Prevention, 2021; 22(6): 1927-1933. doi: 10.31557/APJCP.2021.22.6.1927
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