Early Diagnosis of Oral Squamous Cell Carcinoma (OSCC) by miR-138 and miR-424-5p Expression as a Cancer Marker

Document Type : Research Articles

Authors

1 Craniomaxillofacial Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

2 Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

3 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Department of Biotechnology, School of Advanced Technologies, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Behavioral Sciences Research Center,Shahid Beheshti University of Medical Sciences,Tehran, Iran.

6 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: MicroRNAs (miRs) are a group of endogenous, non-coding, 18–24 nucleotide length single-strand RNAs. These molecules mediate the gene expression and are involved in regulating diverse cellular biological processes, i.e. cell cycle, differentiation, and apoptosis. Aberrant miR expression has been shown to be an important event in the pathologies of various types of cancer, including oral squamous cell carcinoma (OSCC). Methods: Blood samples were obtained from 30 patients (15 cases and 15 controls), to determine miR-138 and miR-424-5p expression by using real-time PCR and ΔΔCT. Results: The median CT values of miR-138 were 27.60 and 28.70, while those of miR 424-5p were 29.40 and 30.0 in the case and control groups, respectively. Mann-Whitney test indicated no significant difference in miR-138 and miR-424-5p between the two groups (P > 0.05). However, results obtained by ΔΔCT method showed that miR-424-5p expression was 1.96 times higher in the case group, but miR-138 expression was 3.05 times lower in the plasma of OSCC patients. Conclusion: Our findings suggest that the evaluation of miR-138 and miR-424-5p expression in serum can be used as potent markers for carcinoma detection and also may be a potentially therapeutic approach in the future. Further longitudinal studies with larger samples are required to verify these findings.
 

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Volume 22, Issue 7
July 2021
Pages 2185-2189
  • Receive Date: 26 April 2021
  • Revise Date: 01 June 2021
  • Accept Date: 23 July 2021
  • First Publish Date: 23 July 2021