Objective: Breast cancer is the most common case of cancers. Apitheraphy has been traditionally used for abundance diseases. This study aims to evaluate and compare the anti-breast cancer activity of melittin from Indonesia’s Apic cerana as a potential drug for treating breast cancer. Methods: Apis cerana bee venom (BV) was collected from a bee farm in Cikurutung, Bandung using an electrical venom device. The BV was then purified using the ÄKTA Start system and HiTrap™ SP HP cation exchange chromatography column. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify melittin based on its molecular mass and lowry’s protein assay to measure melittin concentration. Melittin cytotoxicity was measured with brine shrimp lethality test (BSLT), while MCF-7 breast cancer cells MTT assay was used to measure its anti-breast cancer activity, based on inhinition rate. Results: 95.432 μg/mL melittin is purified from 62.8 mg/L BV, using cation exchange chromatography. Melittin in vitro analysis with MCF-7 MTT assay is used to determine anti-breast cancer activity in dose dependent manner. Furthermore, melttin BSLT result showed a LC50 16.67675 μg/mL. Therefore, the MTT assay was conducted in 5, 10 and 15 μg/mL with MCF-7 inhibition values of 0.768 ± 0.014, 3.303 ± 0.011, and 35.714 ± 0.009 %, respectively. Conclusion: Indonesia’s Apis cerana has the potential to be used as a therapeutic peptide for breast cancer treatment.
Lischer, K., Sitorus, S., Guslianto, B., Avila, F., Khayrani, A., & Sahlan, M. (2021). Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study. Asian Pacific Journal of Cancer Prevention, 22(12), 3913-3919. doi: 10.31557/APJCP.2021.22.12.3913
MLA
Kenny Lischer; Shania Rosita Angelica Sitorus; Brian Wirawan Guslianto; Forbes Avila; Apriliana Cahya Khayrani; Muhamad Sahlan. "Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study". Asian Pacific Journal of Cancer Prevention, 22, 12, 2021, 3913-3919. doi: 10.31557/APJCP.2021.22.12.3913
HARVARD
Lischer, K., Sitorus, S., Guslianto, B., Avila, F., Khayrani, A., Sahlan, M. (2021). 'Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study', Asian Pacific Journal of Cancer Prevention, 22(12), pp. 3913-3919. doi: 10.31557/APJCP.2021.22.12.3913
VANCOUVER
Lischer, K., Sitorus, S., Guslianto, B., Avila, F., Khayrani, A., Sahlan, M. Anti-Breast Cancer Activity on MCF-7 Cells of Melittin from Indonesia’s Apis cerana: An In Vitro Study. Asian Pacific Journal of Cancer Prevention, 2021; 22(12): 3913-3919. doi: 10.31557/APJCP.2021.22.12.3913
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