Association between miR-196a-2 Gene Polymorphism and Ovarian Cancer Prognosis in Egyptian Females

Hussein, Samia; Lasheen, Ahmed El Shabrawy; Abdelrahman, Amr A; Al-Karamany, Amira S; Sameh, Reham; Algazeery, Ahmed

doi:10.31557/APJCP.2022.23.5.1761

Association between miR-196a-2 Gene Polymorphism and Ovarian Cancer Prognosis in Egyptian Females

Document Type : Research Articles

Authors

¹ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

² Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

³ Obstetric and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

⁴ Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

10.31557/APJCP.2022.23.5.1761

Abstract

Background: Ovarian cancer is the fifth leading cause of cancer-related deaths among women worldwide. Unfortunately, early detection tests are relatively lacking. Diagnosis in the late stages of the disease carries a poor prognosis. Objective: To evaluate the relationship between miR-196a-2 rs11614913 polymorphism and ovarian cancer risk and prognosis in Egyptian females. Methods: In this case-control study, the participants were classified into 2 groups. Group A is the control group which included 50 healthy females. Group B included 50 patients newly diagnosed with ovarian carcinoma confirmed by histopathological analysis. Immunohistochemistry for P53 and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for miR-196a-2 genotypes detection were performed. Results: There was a statistically significant difference among ovarian cancer cases and controls regarding genotypes (P = 0.003). However, the distribution of the T and C alleles in both studied groups showed no significant difference (P = 0.17). There was a statistically significant increase of CA 125 levels among CT and CC genotypes carriers of ovarian cancer cases (p = 0.04). Besides, there was a statistically significant correlation between miR-196a-2 polymorphism and each of tumor grade (P <0.001), p53 immunohistochemical expression (P= 0.002), and Figo classification (P <0.001). Conclusion: There was a statistically significant increase of CA 125 levels among C allele carriers of ovarian cancer cases. Besides, there was a statistically significant association between the miR-196a-2 polymorphism and each of tumor grade, p53 immunohistochemical expression, and Figo classification. So, miR-196a-2 polymorphism can be a possible prognostic factor in ovarian cancer.

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